| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.213763 |
| Chromosome: | chromosome_10 |
| Location: | 4622334 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g452950 | TRP1 | Transient receptor potential ion channel protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCTTGCCCACCCAGCAGCGGGTGGTGTGCA |
| Internal bar code: | TTAAATGACGAATCGGATATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 599 |
| LEAP-Seq percent confirming: | 99.6354 |
| LEAP-Seq n confirming: | 10931 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTACGGCAACCAGTACA |
| Suggested primer 2: | CAGGTCCCTCTCCAACAGAG |