| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.213767 |
| Chromosome: | chromosome 5 |
| Location: | 1873259 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241650 | (1 of 1) K01303 - acylaminoacyl-peptidase [EC:3.4.19.1] (APEH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTATCCAATCAATGACTAGCGCGTTAAG |
| Internal bar code: | TTTCTACGATCTTTACCCAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 636 |
| LEAP-Seq percent confirming: | 99.7392 |
| LEAP-Seq n confirming: | 3060 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCGGTACTTGATTGGTG |
| Suggested primer 2: | CGTGGTACATATGGGCACTG |