Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.213776 |
Chromosome: | chromosome_3 |
Location: | 6666124 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g197250 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTAAGCGGATCCAGCTGTCGCAACTCCGCA |
Internal bar code: | AACGGTGTTACCGCGCGGGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 824 |
LEAP-Seq percent confirming: | 84.4726 |
LEAP-Seq n confirming: | 9787 |
LEAP-Seq n nonconfirming: | 1799 |
LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCGCCGTATTCAAAAGTTG |
Suggested primer 2: | GGACAGTAAAGCTTCAGGCG |