| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213782 |
| Chromosome: | chromosome 5 |
| Location: | 1632755 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243150 | Carbonic anhydrase-related protein; (1 of 1) PF04357 - Family of unknown function (DUF490) (DUF490) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCTCTCGGCCCGCGACCAAAAACTGCAA |
| Internal bar code: | TTCGTCTGAGGCACCCAGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 698 |
| LEAP-Seq percent confirming: | 93.8416 |
| LEAP-Seq n confirming: | 320 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTACTTTCGGTGTGGGT |
| Suggested primer 2: | TCTGCAACAAACTACAGGCG |