| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213792 |
| Chromosome: | chromosome 12 |
| Location: | 5282359 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528700 | TSA1,TRPA1,WSA1,WSN1 | Tryptophan synthetase alpha subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCATGTATGGCCGTGGATAAGGATTCA |
| Internal bar code: | GTGGTTCTCCGACAGCGCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 833 |
| LEAP-Seq percent confirming: | 99.349 |
| LEAP-Seq n confirming: | 5341 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCTCTCCGGTAAGTGAG |
| Suggested primer 2: | TACCCCTATACGCTTCCACG |