| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.213811 |
| Chromosome: | chromosome 12 |
| Location: | 2632395 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504550 | HTB15 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTCTGGGGGCACTGGGCTAGGAGGGGGC |
| Internal bar code: | TTGCGTTGACCTGCGCGTCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 202 |
| LEAP-Seq percent confirming: | 99.0514 |
| LEAP-Seq n confirming: | 1253 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGCCCATTATCACAACG |
| Suggested primer 2: | AGCAGAAAGAGGTGAACGGA |