| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213817 |
| Chromosome: | chromosome 13 |
| Location: | 2153973 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578150 | RRAA1,RRA1 | Regulator of ribonuclease activity A; (1 of 1) 4.1.1.3 - Oxaloacetate decarboxylase / Oxaloacetate carboxy-lyase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAATTTGGCGATGGCACACCTTGTCAAC |
| Internal bar code: | CACTGGCTGTCGAATCGCCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1020 |
| LEAP-Seq percent confirming: | 99.9199 |
| LEAP-Seq n confirming: | 4989 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGTGCGTACAACAGAGA |
| Suggested primer 2: | CGCCTTGCTTTACAGCCTAC |