| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.213834 |
| Chromosome: | chromosome 2 |
| Location: | 7186276 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146300 | (1 of 2) PTHR10027 - CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGGCGCGCACTCGGTGCCTGCGACGCC |
| Internal bar code: | TAAGGACGTAAAGGTATAACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 38 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCTGATGACTGTCCAAAG |
| Suggested primer 2: | CTGCTGGTGCATCGTACAGT |