| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.213846 |
| Chromosome: | chromosome 1 |
| Location: | 5446438 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038250 | SDC1 | Serine decarboxylase; (1 of 1) 4.1.1.22 - Histidine decarboxylase / L-histidine carboxy-lyase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCAAAAACCACCACGCTCCACCTCTTCG |
| Internal bar code: | CCGATCACGGAGCTCCCGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 397 |
| LEAP-Seq percent confirming: | 99.5243 |
| LEAP-Seq n confirming: | 3766 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGACCCTTGCAGTTTCTC |
| Suggested primer 2: | ACGCACTTGCCTGAGTTCTT |