| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213867 |
| Chromosome: | chromosome 10 |
| Location: | 5580973 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459800 | (1 of 14) PF00566 - Rab-GTPase-TBC domain (RabGAP-TBC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAATGCATGTACCCCCCACACACACACAC |
| Internal bar code: | TCCTAGTAGACTGAGCGGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 99.4186 |
| LEAP-Seq n confirming: | 1539 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTACATGGGTAGCCAAGCC |
| Suggested primer 2: | TCCAGCTTGCTCAACTTCCT |