| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.213875 |
| Chromosome: | chromosome 12 |
| Location: | 8269855 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGGCGTATGGTACGGTGGGCACTGGGC |
| Internal bar code: | ATTCCAGAGACGGGTCATGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 912 |
| LEAP-Seq percent confirming: | 97.4648 |
| LEAP-Seq n confirming: | 8650 |
| LEAP-Seq n nonconfirming: | 225 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAAGCTACTGGAGGCACC |
| Suggested primer 2: | TTTGATGCAGGGTGTCATGT |