| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.213878 |
| Chromosome: | chromosome 1 |
| Location: | 1970218 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010650 | (1 of 1) PF12530 - Protein of unknown function (DUF3730) (DUF3730) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTTAATTAAGAAATGAAGTCGTAGGCC |
| Internal bar code: | TCTGGTTTGCCGGGGGCACGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 276 |
| LEAP-Seq percent confirming: | 99.9107 |
| LEAP-Seq n confirming: | 2238 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGCGTACAGCTCAAGAC |
| Suggested primer 2: | TTGATCAAGCATACGGCAAG |