Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.213878 |
Chromosome: | chromosome_2 |
Location: | 600528 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g077800 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGAAGTGTGAGCGTAGGCGCGCGCGCCGGC |
Internal bar code: | TGGGGTTGATCTGCACCGGGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 875 |
LEAP-Seq percent confirming: | 99.7949 |
LEAP-Seq n confirming: | 1460 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTGGATGTGCGCTAGTTTG |
Suggested primer 2: | GGACATGACATCCACTGCAC |