Insertion junction: LMJ.RY0402.213879_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.213879
Chromosome:chromosome 12
Location:3345287
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g497450 FAP412 (1 of 21) IPR001611//IPR003591 - Leucine-rich repeat // Leucine-rich repeat, typical subtype; Flagellar Associated Protein 412 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGAGGGGGAGGGGAAGGGATATATGGTAT
Internal bar code:GACACAGGCCCGCGCGAAGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:334
LEAP-Seq percent confirming:97.2763
LEAP-Seq n confirming:250
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TACCAGCCCAAACTAAACCG
Suggested primer 2:CTTAGACCTGCATTCCCGAG