Insertion junction: LMJ.RY0402.213880_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.213880
Chromosome:chromosome 13
Location:2022082
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g576850 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGAATGTGCGAGACTTCCGTAGCACACTT
Internal bar code:GCGAAATAGTACTAGGGTAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:122
LEAP-Seq percent confirming:99.4012
LEAP-Seq n confirming:332
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGGTTTACTTTGGGCTGG
Suggested primer 2:GAGAACAAGAAGCGGAGGTG