| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213890 |
| Chromosome: | chromosome 1 |
| Location: | 332675 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001983 | C2-domain containing protein; (1 of 2) PTHR32246//PTHR32246:SF18 - FAMILY NOT NAMED // C2 DOMAIN-CONTAINING PROTEIN-RELATED | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGTAAAGGGGCCGGATGCGCTTCGCCG |
| Internal bar code: | GGGCTGTTGTAGTATCGGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 872 |
| LEAP-Seq percent confirming: | 99.8181 |
| LEAP-Seq n confirming: | 2195 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCACCCCCTTCTTAAATA |
| Suggested primer 2: | GATGCCCGTTGTCGTCTATT |