| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213891 |
| Chromosome: | chromosome 12 |
| Location: | 1618351 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486350 | STPK3,STK3 | (1 of 3) K07359 - calcium/calmodulin-dependent protein kinase kinase (CAMKK); Serine/threonine protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTGTTACGGAGCCAATGCGGCGGGGAGA |
| Internal bar code: | GGCACATGCCCAAGGGGCGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 587 |
| LEAP-Seq percent confirming: | 97.9381 |
| LEAP-Seq n confirming: | 1425 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGCTTACTAGTTGCTCGC |
| Suggested primer 2: | ACGCAGTTTACCACAGGTCC |