| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.213893 |
| Chromosome: | chromosome 12 |
| Location: | 6900416 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560700 | MDJ1B,CDJ6 | (1 of 2) PTHR24078//PTHR24078:SF290 - DNAJ HOMOLOG SUBFAMILY C MEMBER // SUBFAMILY NOT NAMED; Mitochondrial DnaJ-like protein 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGGTAAGTAAAGCCTCCCAGACCATACT |
| Internal bar code: | TGACTGCGTATCATGTTGTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.7937 |
| LEAP-Seq n confirming: | 1451 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATACGGGGTGGGTAGGTT |
| Suggested primer 2: | ACGGAACCCAGACGATAGTG |