Insertion junction: LMJ.RY0402.213906_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.213906
Chromosome:chromosome 9
Location:6236544
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGGGGGGCCTCGCTGCTGCCTTCGTCAA
Internal bar code:AAGTACGACGGTTTAGGAGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:485
LEAP-Seq percent confirming:97.999
LEAP-Seq n confirming:2057
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CACTTCCCTGAACGGTTTGT
Suggested primer 2:GAGGGTGGCTGTCAAGTTGT