| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.213921 |
| Chromosome: | chromosome 10 |
| Location: | 1484348 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428720 | (1 of 41) PTHR32523//PTHR32523:SF8 - FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCACACGCTGCCTGCCCCGGCTGCGGC |
| Internal bar code: | GCACTCGGATCGACGAGTCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 37 |
| LEAP-Seq percent confirming: | 5.51091 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 823 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAAGTTGCGAGTGTGCAA |
| Suggested primer 2: | AGGTACGTGCCACAATGTCA |