Insertion junction: LMJ.RY0402.213929_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.213929
Chromosome:chromosome 7
Location:4806466
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g345750 NAT20 N-acetyltransferase; (1 of 3) IPR000182//IPR011011//IPR016181 - GNAT domain // Zinc finger, FYVE/PHD-type // Acyl-CoA N-acyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCTTGATTCGACGTCACTGCCTCCACTG
Internal bar code:GCGTTTTGAAGGAACTCCATA

Confirmation - LEAP-Seq

LEAP-Seq distance:186
LEAP-Seq percent confirming:15.5606
LEAP-Seq n confirming:136
LEAP-Seq n nonconfirming:738
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGGGCGCAGTCGAAGTAG
Suggested primer 2:CAGCTTTCCCCTTATCTCCC