| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.213930 |
| Chromosome: | chromosome 7 |
| Location: | 1131375 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g320350 | CDJ5 | (1 of 3) PF00226//PF13370 - DnaJ domain (DnaJ) // 4Fe-4S single cluster domain of Ferredoxin I (Fer4_13); Chloroplast DnaJ-like protein 5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAGTTGTCCCGGACGTGGGAGCAAAGTG |
| Internal bar code: | GGGCGATGATCCTTTTTAAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 869 |
| LEAP-Seq percent confirming: | 99.2545 |
| LEAP-Seq n confirming: | 3994 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCACAACCGCCTCTAAG |
| Suggested primer 2: | AGTCGGACGTTTATGTTGGC |