| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.213947 |
| Chromosome: | chromosome 3 |
| Location: | 4629087 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177300 | HAP4 | (1 of 3) 1.11.1.10 - Chloride peroxidase / Vanadium haloperoxidase; Haloperoxidase-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTGTGCGCGACTATAAGAAGGCTAAGCT |
| Internal bar code: | GTATTCTATGAGCGATCAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 134 |
| LEAP-Seq percent confirming: | 96.2054 |
| LEAP-Seq n confirming: | 431 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACAAGGCCTGCGTGTTTC |
| Suggested primer 2: | ACGCATGAAAGGGCATAAAG |