Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.213970 |
Chromosome: | chromosome 17 |
Location: | 2025300 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATCATCATCATGCGCATCCACTACTGAA |
Internal bar code: | GGGTACGCTTGTGGAAAGGGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 812 |
LEAP-Seq percent confirming: | 99.5623 |
LEAP-Seq n confirming: | 18426 |
LEAP-Seq n nonconfirming: | 81 |
LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCACCAGGGGTATTACGATG |
Suggested primer 2: | TGCTCTAGCACGTCACCATC |