Insertion junction: LMJ.RY0402.214029_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.214029
Chromosome:chromosome 8
Location:2375059
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g370900 (1 of 6) PTHR22870//PTHR22870:SF201 - REGULATOR OF CHROMOSOME CONDENSATION // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCCGTACGGCATCCAACATTCGATACGT
Internal bar code:GAGGCTCGTCGATTTGCCAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:753
LEAP-Seq percent confirming:99.7868
LEAP-Seq n confirming:936
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTAGGCTGTCGTGTTGTT
Suggested primer 2:ACATGCTGAATGCTAAGGGC