Insertion junction: LMJ.RY0402.214039_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.214039
Chromosome:chromosome 12
Location:7882002
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g552750 (1 of 2) PF04520 - Senescence regulator (Senescence_reg) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTGGCGCGTGTGTGTGCTGGAGCTGCCC
Internal bar code:GGAACGGACTTCGAGGATAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:894
LEAP-Seq percent confirming:95.4867
LEAP-Seq n confirming:13096
LEAP-Seq n nonconfirming:619
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGCATATAGGGCCTGTG
Suggested primer 2:ACTCATTCATACCCCACCCA