| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214040 |
| Chromosome: | chromosome 7 |
| Location: | 525411 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g316150 | (1 of 1) PTHR13146//PTHR13146:SF0//PTHR32523//PTHR32523:SF8 - UNCHARACTERIZED // SOLUTE CARRIER FAMILY 35 MEMBER F6 // FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGCAGCTCAGATGTGCTCTAGGGGCGG |
| Internal bar code: | AGGTGCCCCAGAGACAGTTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 607 |
| LEAP-Seq percent confirming: | 99.1788 |
| LEAP-Seq n confirming: | 4831 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGCCTGTTTGGTGAGGT |
| Suggested primer 2: | TCGAGCTCCTCCTCCTCATA |