Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.214050 |
Chromosome: | chromosome 3 |
Location: | 8687545 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g203793 | GT90-35,GT90F35 | (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 35 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCTGGGACTTTGGCAATGTCGTCCAAAG |
Internal bar code: | GAAATCCGGACCCATTGGCCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 823 |
LEAP-Seq percent confirming: | 99.6483 |
LEAP-Seq n confirming: | 7366 |
LEAP-Seq n nonconfirming: | 26 |
LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGGGGTTGTTTTATTCCCAC |
Suggested primer 2: | GTCTCAGTGCCTGCCATGTA |