| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.214055 |
| Chromosome: | chromosome 4 |
| Location: | 1957894 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTACGACAGGCGGCCGGCCCTCCCGATCC |
| Internal bar code: | TAATACCAAGGGGTTAATGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 108 |
| LEAP-Seq percent confirming: | 99.8198 |
| LEAP-Seq n confirming: | 554 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGCCTCTGACGAGTCCTA |
| Suggested primer 2: | ACGCTAGCCCATGCATTTAC |