Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.214058 |
Chromosome: | chromosome_11 |
Location: | 331284 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g467567 | UBQ1 | Ubiquitin | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCGCGGCGCTTGTCAAGATGCAGATTTTCG |
Internal bar code: | ACAGGGCTCAGCTACGGGGGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 631 |
LEAP-Seq percent confirming: | 99.6703 |
LEAP-Seq n confirming: | 907 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCGGATTCCGTGATCTTGT |
Suggested primer 2: | CCATTAAGGCGGTGAGAAAA |