| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.214075 |
| Chromosome: | chromosome 4 |
| Location: | 1167970 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215750 | (1 of 1) K02180 - cell cycle arrest protein BUB3 (BUB3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTTCGACGCCGCAGACGCGCAGGTGCG |
| Internal bar code: | GGCCCGGCAACAGGGCCCTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 834 |
| LEAP-Seq percent confirming: | 99.7328 |
| LEAP-Seq n confirming: | 6719 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTCTCACAGAAGCTCCCC |
| Suggested primer 2: | GCTGCCCTCAATGTGTACCT |