| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.214077 |
| Chromosome: | chromosome 9 |
| Location: | 2781299 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389050 | CNX5,MAW4 | (1 of 2) PTHR16861//PTHR16861:SF4 - GLYCOPROTEIN 38 // PODOPLANIN; Membrane-associated hydroxyproline-rich glycoprotein 4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTTCACGCATGGGGCGGCAGGTTGCAA |
| Internal bar code: | TGCCCCCGGGTTAGCTTTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1031 |
| LEAP-Seq percent confirming: | 99.5781 |
| LEAP-Seq n confirming: | 1416 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTACTTCATTTGGTGCGT |
| Suggested primer 2: | GTGCTGGGCTAGGAGGATTC |