Insertion junction: LMJ.RY0402.214077_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.214077
Chromosome:chromosome 9
Location:2781307
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g389050 CNX5,MAW4 (1 of 2) PTHR16861//PTHR16861:SF4 - GLYCOPROTEIN 38 // PODOPLANIN; Membrane-associated hydroxyproline-rich glycoprotein 4 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAGCTCAGACCGCCGCAGCACGCCGCTAG
Internal bar code:TTGGCCATCTTCCCCCATTCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:227
LEAP-Seq percent confirming:99.6226
LEAP-Seq n confirming:792
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGACCTGGCTCGGGACTT
Suggested primer 2:CCCTACTTCATTTGGTGCGT