| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.214083 |
| Chromosome: | chromosome 13 |
| Location: | 3578955 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588250 | (1 of 1) K16457 - centrosomal protein CEP76 (CEP76) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTAGGAAAAACCCTCGACGGCGCTCACTC |
| Internal bar code: | CACATGCTAGCTTACCTACTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 426 |
| LEAP-Seq percent confirming: | 99.6774 |
| LEAP-Seq n confirming: | 618 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGAGCCAGGGACAAAAT |
| Suggested primer 2: | GCTGCGTTTAGCGAGCTAGT |