| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214085 |
| Chromosome: | chromosome_16 |
| Location: | 6893169 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g676533 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTACGGCACCAATGACCCGCTTTGCGTCGG |
| Internal bar code: | AGACGAACGGGGTTCTACATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 714 |
| LEAP-Seq percent confirming: | 99.1249 |
| LEAP-Seq n confirming: | 2945 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGTTGCGGCTAGAACAC |
| Suggested primer 2: | ACGCTTAACATGAAGGTGGG |