Insertion junction: LMJ.RY0402.214087_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.214087
Chromosome:chromosome 3
Location:8044639
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g201400 PPP16,PP2C6 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGACCGTGAACCGCTCCGTGTACACCACCA
Internal bar code:TTCGTGCTCGTTGTTTGGCTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:523
LEAP-Seq percent confirming:99.6358
LEAP-Seq n confirming:5198
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTAAGAGCACTGCCTTTGCC
Suggested primer 2:ATACAGAACCGCACCACCTC