Insertion junction: LMJ.RY0402.214126_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.214126
Chromosome:chromosome 13
Location:3194167
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g585500 HAP1 (1 of 12) IPR000326 - Phosphatidic acid phosphatase type 2/haloperoxidase; Putative haloperoxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCATGAAATCCCCGGTGGCCCCATGTG
Internal bar code:GTGAGACGACGTTGTAATCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:752
LEAP-Seq percent confirming:99.8149
LEAP-Seq n confirming:2696
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTGCTGGCGATTATGATT
Suggested primer 2:ATTGCATCCTCCTCCTCCTT