| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214165 |
| Chromosome: | chromosome 8 |
| Location: | 414505 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358579 | (1 of 1) PTHR10984:SF32 - ENDOPLASMIC RETICULUM VESICLE TRANSPORTER PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCTCGCCTTCATAGCACACCTACCCGT |
| Internal bar code: | GAGGCCGACCCGACTTGCGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 441 |
| LEAP-Seq percent confirming: | 99.7755 |
| LEAP-Seq n confirming: | 2222 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCCTAGCGCCTCTGTAG |
| Suggested primer 2: | GGTGGACTTATGGATGTGCC |