| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.214170 |
| Chromosome: | chromosome 15 |
| Location: | 1140747 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641100 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAGAAGGAGCGAAGCTGCCTGCAAACTG |
| Internal bar code: | TCCGCGCCTGGGAGTGGAACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 916 |
| LEAP-Seq percent confirming: | 99.604 |
| LEAP-Seq n confirming: | 3773 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCATGTAGAGCTGATGCAA |
| Suggested primer 2: | TTATACCCCGAAAGCGTGTC |