| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.214170 |
| Chromosome: | chromosome 15 |
| Location: | 1140751 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641100 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCCGAAGCTATCCGGTTTGGTTTAGAA |
| Internal bar code: | CTTACATCGTCCAAAGAGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 398 |
| LEAP-Seq percent confirming: | 91.5858 |
| LEAP-Seq n confirming: | 283 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCATGTAGAGCTGATGCAA |
| Suggested primer 2: | TTATACCCCGAAAGCGTGTC |