| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.214173 |
| Chromosome: | chromosome 10 |
| Location: | 3504238 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g445100 | CGL50 | (1 of 1) PTHR13068:SF26 - MITOCHONDRIAL TRANSCRIPTION TERMINATION FACTOR FAMILY PROTEIN; Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTTCCATTCTTGTCCTGTGCAGGGGGC |
| Internal bar code: | CGACTTCACACGTCGTGGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1042 |
| LEAP-Seq percent confirming: | 90.6488 |
| LEAP-Seq n confirming: | 4876 |
| LEAP-Seq n nonconfirming: | 503 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAATAGAGTGCTGCGGTG |
| Suggested primer 2: | AGGTTGGTTTCGCCAGTATG |