| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.214175 |
| Chromosome: | chromosome 2 |
| Location: | 7916038 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141200 | NIC2 | Quinolinate phosphoribosyl transferase; (1 of 1) 2.4.2.19 - Nicotinate-nucleotide diphosphorylase (carboxylating) / Quinolinate phosphoribosyltransferase (decarboxylating) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCACCCACTCTCTCCTGCCATGCGCGT |
| Internal bar code: | AGGACGGTATTCCAGTACCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 753 |
| LEAP-Seq percent confirming: | 69.1341 |
| LEAP-Seq n confirming: | 3026 |
| LEAP-Seq n nonconfirming: | 1351 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGTTGGCGATAGTGGAC |
| Suggested primer 2: | AAAATCGCATCACTTCGGAC |