Insertion junction: LMJ.RY0402.214198_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.214198
Chromosome:chromosome 8
Location:1964109
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g367750 DIV144,GT90-12,GT90F12 GT90 family protein 12; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTGCACTGGTGGTGGGAGATTGCACTGGG
Internal bar code:AAGGTTCCGTCGGAATCAAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1004
LEAP-Seq percent confirming:95.0746
LEAP-Seq n confirming:3822
LEAP-Seq n nonconfirming:198
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CCTACCGCTATCTCAGCCAG
Suggested primer 2:AATCAAGAAGCTTCCCAGCA