Insertion junction: LMJ.RY0402.214198_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.214198
Chromosome:chromosome 8
Location:1964186
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g367750 DIV144,GT90-12,GT90F12 GT90 family protein 12; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGACTTCTCATAGTCCGCACACCCAAAT
Internal bar code:CGTGGGCAGGTACTTGATGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:847
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCTACCGCTATCTCAGCCAG
Suggested primer 2:AATCAAGAAGCTTCCCAGCA