Insertion junction: LMJ.RY0402.214201_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.214201
Chromosome:chromosome 5
Location:798422
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre05.g248200 (1 of 2) PTHR21493//PTHR21493:SF124 - CGI-141-RELATED/LIPASE CONTAINING PROTEIN // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAACCGCCGGCAATCAATCTGCCAATGG
Internal bar code:CACGGAACTAACGATCGCTAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:904
LEAP-Seq percent confirming:99.7753
LEAP-Seq n confirming:3108
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGCCTTTCTTCCTTGCTT
Suggested primer 2:GCGATCACACGAGAAGACAA