| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214201 |
| Chromosome: | chromosome 6 |
| Location: | 8703238 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g309750 | (1 of 1) PTHR12458//PTHR12458:SF7 - ORF PROTEIN // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGCACCCCTTATCTTACGGCGTGCTGAT |
| Internal bar code: | TCACGTGTCGGGCTGCTACCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 622 |
| LEAP-Seq percent confirming: | 97.2263 |
| LEAP-Seq n confirming: | 666 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGGTGGAAGTGTGTGC |
| Suggested primer 2: | CTCTCGCTGAACCTATTCCG |