| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214225 |
| Chromosome: | chromosome 10 |
| Location: | 3625504 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g445600 | (1 of 1) K14569 - ribosome biogenesis protein BMS1 (BMS1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTCAGCAATCTCTAGCACCAAGGGCACA |
| Internal bar code: | CTGAGCCGCACGCGTAATATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 753 |
| LEAP-Seq percent confirming: | 99.4429 |
| LEAP-Seq n confirming: | 1428 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAGTGCTCCAGCTTAGCC |
| Suggested primer 2: | GTATGAGAAGAAGCAGGCCG |