Insertion junction: LMJ.RY0402.214243_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.214243
Chromosome:chromosome 7
Location:4909228
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g346317 GT90F39,GT90-39 GT90 family protein 39; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTCGATCCATTGGGCCGCGGCGCCGCCGG
Internal bar code:AGAGGCTCAGGACCGCTACCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:384
LEAP-Seq percent confirming:99.8343
LEAP-Seq n confirming:1205
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TAGTACTCCGCCCAGTTGCT
Suggested primer 2:GCCACACACATGCACACATA