| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214253 |
| Chromosome: | chromosome 7 |
| Location: | 2272120 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327687 | (1 of 1) PF06080 - Protein of unknown function (DUF938) (DUF938) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAACCTTCGCAACCTTTGTTTATCTTCA |
| Internal bar code: | TTGTCTGAGTGCGTGCTGGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 96.3819 |
| LEAP-Seq n confirming: | 959 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGCTCCTGTCAATAACG |
| Suggested primer 2: | TATGTAAGGTGGCTCAGGGC |