| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.214267 |
| Chromosome: | chromosome 8 |
| Location: | 538233 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g359200 | (1 of 6) PTHR22870//PTHR22870:SF201 - REGULATOR OF CHROMOSOME CONDENSATION // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACATTTCAGCAGTTACCGTAGTACGGT |
| Internal bar code: | AATATTCAATTTCTGGGGGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 99.1435 |
| LEAP-Seq n confirming: | 463 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGACAAGAAGAACAGAGG |
| Suggested primer 2: | CTTGCTTGTACGTGTTGCGT |